![]() ![]() Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time. The process is done by either electroblotting or capillary blotting. Although this step determines the name of this technique Southern blotting, the term is typically used to describe the entire procedure. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Blotting refers to the transfer of the fragmented DNA sequence to the nitrocellulose membrane or nylon membrane. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. This process involves the transfer of protein patterns from gel to microporous membrane. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. ![]() Easy-complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody.Excellent stability-kit is stable for 1 year stored at 4☌.Economical-cost as little as $8 per blot no expensive consumables or extra equipment.Simple-optimized protocol makes Western blot analysis easier than ever.Convenient-no expensive hardware or vacuum required no clogging issues. ![]() Fast-all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot.The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes. ![]()
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